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recombinant human il 35  (Sino Biological)


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    Structured Review

    Sino Biological recombinant human il 35
    Recombinant Human Il 35, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il+35/Human+IL-35+(IL12A+%26+EBI3+Heterodimer)+Protein/pm40254928-74-51-58
    Average 91 stars, based on 6 article reviews
    recombinant human il 35 - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Gene Expression and Antiviral Activity of Interleukin-35 in Response to Influenza A Virus Infection
    Article Snippet: .. The following reagents were purchased: antibodies against RNA-dependent protein kinase (PKR), 2’,5’-oligoadenylate synthetase (OAS), myxovirus resistance protein (Mx), GAPDH, NF-κB /p65 and p50, IFNAR1 , IFNLR1, IκB , pIκB and IL-35/EBI3 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); antibodies against IL-35/p35 (R&D Systems, Minneapolis, USA ); a human IL-35 ELISA kit (eBioscience, San Diego, CA, USA); recombinant human IL-35 (rhIL-35, Sino Biological Inc., Beijing, China); prostaglandin E2 (PGE2, Sigma-Aldrich, St. Louis, MO, USA); Sodium Nitroferricyanide(III) Dihydrate(SNP, NO donor, Beyotime, China),and the inhibitor of iNOS, SMT (Alexis Biochemicals, Grünberg, Germany); recombinant human IFNγ (Peprotech, London, UK) ,si-COX-2,si-iNOS and si-p35(Gene Pharma, China). ..

    Article Title: Gene Expression and Antiviral Activity of Interleukin-35 in Response to Influenza A Virus Infection
    Article Snippet: .. The following reagents were purchased: antibodies against PKR, OAS, Mx, GAPDH, NF-κB/p65 and p50, IFNAR1, IFNLR1, IκB, p-IκB, and IL-35/EBI3 (Santa Cruz Biotechnology, Santa Cruz, CA); antibodies against IL-35/p35 (R&D Systems, Minneapolis, MN); a human IL-35 ELISA kit (eBioscience, San Diego, CA); recombinant human IL-35 (rhIL-35, Sino Biological Inc., Beijing, China); prostaglandin E2 (PGE2, Sigma); sodium nitroferricyanide(III) dihydrate (SNP, nitric oxide donor, Beyotime, China), and the inhibitor of iNOS, SMT; recombinant human IFNγ (Peprotech, London, UK), si-COX-2,si-iNOS, and si-p35 (Gene Pharma, China). ..

    Recombinant:

    Article Title: Gene Expression and Antiviral Activity of Interleukin-35 in Response to Influenza A Virus Infection
    Article Snippet: .. The following reagents were purchased: antibodies against RNA-dependent protein kinase (PKR), 2’,5’-oligoadenylate synthetase (OAS), myxovirus resistance protein (Mx), GAPDH, NF-κB /p65 and p50, IFNAR1 , IFNLR1, IκB , pIκB and IL-35/EBI3 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); antibodies against IL-35/p35 (R&D Systems, Minneapolis, USA ); a human IL-35 ELISA kit (eBioscience, San Diego, CA, USA); recombinant human IL-35 (rhIL-35, Sino Biological Inc., Beijing, China); prostaglandin E2 (PGE2, Sigma-Aldrich, St. Louis, MO, USA); Sodium Nitroferricyanide(III) Dihydrate(SNP, NO donor, Beyotime, China),and the inhibitor of iNOS, SMT (Alexis Biochemicals, Grünberg, Germany); recombinant human IFNγ (Peprotech, London, UK) ,si-COX-2,si-iNOS and si-p35(Gene Pharma, China). ..

    Article Title: Gene Expression and Antiviral Activity of Interleukin-35 in Response to Influenza A Virus Infection
    Article Snippet: .. The following reagents were purchased: antibodies against PKR, OAS, Mx, GAPDH, NF-κB/p65 and p50, IFNAR1, IFNLR1, IκB, p-IκB, and IL-35/EBI3 (Santa Cruz Biotechnology, Santa Cruz, CA); antibodies against IL-35/p35 (R&D Systems, Minneapolis, MN); a human IL-35 ELISA kit (eBioscience, San Diego, CA); recombinant human IL-35 (rhIL-35, Sino Biological Inc., Beijing, China); prostaglandin E2 (PGE2, Sigma); sodium nitroferricyanide(III) dihydrate (SNP, nitric oxide donor, Beyotime, China), and the inhibitor of iNOS, SMT; recombinant human IFNγ (Peprotech, London, UK), si-COX-2,si-iNOS, and si-p35 (Gene Pharma, China). ..

    Article Title: Cytokine Expression and Cytolytic Effect of Natural Killer Cells are Suppressed in Septic Shock.
    Article Snippet: Septic shock is the most severe stage of sepsis.. How immune dysregulation contributes to the pathogenesis of septic shock has not been thoroughly understood.. In the current research, the phenotype and function of circulating natural killer (NK) cells of septic patients were characterised.

    Incubation:

    Article Title: Cytokine Expression and Cytolytic Effect of Natural Killer Cells are Suppressed in Septic Shock.
    Article Snippet: Septic shock is the most severe stage of sepsis.. How immune dysregulation contributes to the pathogenesis of septic shock has not been thoroughly understood.. In the current research, the phenotype and function of circulating natural killer (NK) cells of septic patients were characterised.

    Flow Cytometry:

    Article Title: Cytokine Expression and Cytolytic Effect of Natural Killer Cells are Suppressed in Septic Shock.
    Article Snippet: Septic shock is the most severe stage of sepsis.. How immune dysregulation contributes to the pathogenesis of septic shock has not been thoroughly understood.. In the current research, the phenotype and function of circulating natural killer (NK) cells of septic patients were characterised.

    Expressing:

    Article Title: Cytokine Expression and Cytolytic Effect of Natural Killer Cells are Suppressed in Septic Shock.
    Article Snippet: Septic shock is the most severe stage of sepsis.. How immune dysregulation contributes to the pathogenesis of septic shock has not been thoroughly understood.. In the current research, the phenotype and function of circulating natural killer (NK) cells of septic patients were characterised.

    Co-Culture Assay:

    Article Title: Cytokine Expression and Cytolytic Effect of Natural Killer Cells are Suppressed in Septic Shock.
    Article Snippet: Septic shock is the most severe stage of sepsis.. How immune dysregulation contributes to the pathogenesis of septic shock has not been thoroughly understood.. In the current research, the phenotype and function of circulating natural killer (NK) cells of septic patients were characterised.



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    Image Search Results


    Cells were isolated from PBMC (A and B), LN, or spleen (C and D) of control mice (immunized with CFA) or EAU mice (IRBP/CFA immunized mice) on day 21 postimmunization and analyzed by the intracellular cytokine staining assay, FACS analysis of cell surface protein expression or Western blot analysis. (A) The frequency of B cells secreting IL-35 was determined by gating on CD19+ (upper panels) and CD19− (lower panels) lymphocytes. (B) The frequency of B cells secreting both IL-10 and IL-35 was determined by gating on p35-expressing (upper panels) and nonexpressing (lower panels) CD19+Ebi3+ B cells. (C and D) Frequency of IL-35 producing B cells was also determined by gating on CD138+ and B220Lo CD19+ B cell pools. Numbers in quadrants indicate percent of cells expressing the relevant proteins as indicated on Figures. Results represent at least 3 independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001)

    Journal: Journal of leukocyte biology

    Article Title: Production of IL-35 by Bregs is mediated through binding of BATF-IRF-4-IRF-8 complex to il12a and ebi3 promoter elements

    doi: 10.1002/JLB.3A0218-071RRR

    Figure Lengend Snippet: Cells were isolated from PBMC (A and B), LN, or spleen (C and D) of control mice (immunized with CFA) or EAU mice (IRBP/CFA immunized mice) on day 21 postimmunization and analyzed by the intracellular cytokine staining assay, FACS analysis of cell surface protein expression or Western blot analysis. (A) The frequency of B cells secreting IL-35 was determined by gating on CD19+ (upper panels) and CD19− (lower panels) lymphocytes. (B) The frequency of B cells secreting both IL-10 and IL-35 was determined by gating on p35-expressing (upper panels) and nonexpressing (lower panels) CD19+Ebi3+ B cells. (C and D) Frequency of IL-35 producing B cells was also determined by gating on CD138+ and B220Lo CD19+ B cell pools. Numbers in quadrants indicate percent of cells expressing the relevant proteins as indicated on Figures. Results represent at least 3 independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001)

    Article Snippet: Briefly, activated B cells were stimulated with recombinant mouse IL-35 4 or human recombinant IL-35 (rhIL-35 [Enzo Life Science, Farmingdale, NY], 20 ng/ml), and DNA-protein complexes were cross-linked for 10 min by addition of fresh formaldehyde (Sigma) to the culture medium at a final concentration of 1%, followed by quenching in 135 mM glycine.

    Techniques: Isolation, Control, Staining, Expressing, Western Blot

    To characterize inflammatory cells that cross the blood–retina barrier during EAU, retinas from control and EAU mice were minced, digested with collagenase and the released cells were subjected to flow cytometry analysis. (A) We analyzed total retinal cell mixture for cell surface CD4 or CD19 expression by FACS. (B) CD4-gated cells were analyzed by intracellular cytokine staining assay and numbers in the quadrants are percentage of CD4 cells expressing IL-2, IL-10, IL-17, IFN-γ, or Foxp3. (C) CD19+ B cells in the retina were analyzed by intracellular cytokine staining assay for expression of IL-10 or IL-35 (p35 & Ebi3). (D–F) We analyzed cells present in the retina, spleen, or LN during EAU for the expression of chemokine receptors and cell surface proteins that characterize the Breg phenotype by FACS. Numbers in quadrants indicate percent of cells expressing the relevant proteins as indicated on Figures. The experiments were performed with at least 6 mice per group and results represent at least 3 independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)

    Journal: Journal of leukocyte biology

    Article Title: Production of IL-35 by Bregs is mediated through binding of BATF-IRF-4-IRF-8 complex to il12a and ebi3 promoter elements

    doi: 10.1002/JLB.3A0218-071RRR

    Figure Lengend Snippet: To characterize inflammatory cells that cross the blood–retina barrier during EAU, retinas from control and EAU mice were minced, digested with collagenase and the released cells were subjected to flow cytometry analysis. (A) We analyzed total retinal cell mixture for cell surface CD4 or CD19 expression by FACS. (B) CD4-gated cells were analyzed by intracellular cytokine staining assay and numbers in the quadrants are percentage of CD4 cells expressing IL-2, IL-10, IL-17, IFN-γ, or Foxp3. (C) CD19+ B cells in the retina were analyzed by intracellular cytokine staining assay for expression of IL-10 or IL-35 (p35 & Ebi3). (D–F) We analyzed cells present in the retina, spleen, or LN during EAU for the expression of chemokine receptors and cell surface proteins that characterize the Breg phenotype by FACS. Numbers in quadrants indicate percent of cells expressing the relevant proteins as indicated on Figures. The experiments were performed with at least 6 mice per group and results represent at least 3 independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)

    Article Snippet: Briefly, activated B cells were stimulated with recombinant mouse IL-35 4 or human recombinant IL-35 (rhIL-35 [Enzo Life Science, Farmingdale, NY], 20 ng/ml), and DNA-protein complexes were cross-linked for 10 min by addition of fresh formaldehyde (Sigma) to the culture medium at a final concentration of 1%, followed by quenching in 135 mM glycine.

    Techniques: Control, Flow Cytometry, Expressing, Staining